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Products - TNT Agar Biplates

Phone Orders: 1-877-398-1360
Email Orders: media@udderhealth.com
BA_plate

 

 

Purpose:

This biplate is used at Udder Health Systems Laboratory for selection and identification of both gram-positive and gram-negative organisms.

Description:

• The bright red agar portion of the biplate is prepared from a modified Columbia base agar with the addition of 5% defibrinated sheep blood, and esculin. This selective agar has been formulated to enhance the growth of gram-positive staphylococci, streptococci and enterococci bacteria while inhibiting the growth of all gram-negative organisms, such as Enterobacteriaceae and Pseudomonas. The addition of esculin enhances the capability of differentiating Streptococci. Certain Streptococci will hydrolyze esculin, causing a mild background blackening. This blackening differentiates E-streps from Streptococcus agalactiae which cannot split esculin. All suspect Streptococcus agalactiae organisms should be moved to our special Camp media for confirmation.

• The pink agar portion of the biplate is specialized media for the detection of gram-negative organisms. Since the antimicrobial agents in this agar inhibit the growth of gram-positive bacteria, the media focuses on the organisms’ ability to ferment lactose. This fermentation causes the bacterial colony to change to a pinkish color. Non-lactose fermenting colonies will remain colorless and opaque.

TNT Agar Biplates - Culture Response:

 

Gram Positive Agar

MacConkey Agar

 Organism

Growth

Reaction

Growth

Reaction

Strep. agalactiae

Growth

Esculin Negative

Inhibited

 

Staph. aureus

Growth

Hemolytic Variable

Inhibited

 

Mycoplasma

Not Applicable

 

Not Applicable

 

Strep. dysgalactiae

Growth

Esculin Variable

Inhibited

 

Strep. uberis

Growth

Esculin Positive

Inhibited

 

E- strep

Growth

Esculin Positive

Inhibited

 

Staph. species

Growth

Hemolytic Variable

Inhibited

 

E. Coli

Inhibited

 

Excellent

Pink Colonies

Klebsiella pneumoniae

Inhibited

 

Excellent

Pink Mucoid Colonies

Klebsiella species

Inhibited

 

Excellent

Pink Mucoid Colonies

Pseudomonas species

Inhibited

 

Excellent

Colorless Colonies

Pseudomonas aeruginosa

Inhibited

 

Excellent

Green/Colorless Colonies

Pasteurella

Weak

 

No Growth

 

Proteus

Ínhibited

 

No Growth

 

Serratia

Inhibited

 

Excellent

Pinkish/Red Pigment

Bacillus

Inhibited

 

Inhibited

 

Yeast

Not Applicable

 

No Growth

 

Mold

Not Applicable

 

Not Applicable

 

Nocardia

Not Applicable

 

Not Applicable

 

Prototheca

Not Applicable

 

Not Applicable

 

Trueperella pyogenes

Growth

Small Hemolytic

Inhibited

 

C. bovis

Not Applicable

 

Not Applicable

 


Other Products:

Blood Agar Plate

This is the standard isolation agar used at Udder Health Systems Laboratory for cultivating a wide range of mastitis organisms from individual cow and bulk tank milk samples.

Inulin Agar Plate

This is an indicator agar used for differentiating Streptococcus uberis from other E-streps in isolates from individual cow samples.  The agar is also used as a selective agar in bedding samples for the isolation of Streptococcus uberis.

MacConkey Agar

This agar is used at Udder Health Systems Laboratory for the detection, selection, and identification of various gram negative organisms from individual cow and environmental samples.

Camp Agar Plate

This is a specialized indicator agar used at Udder Health Systems Laboratory for confirmation of Streptococcus agalactiae suspects.

Salt Agar Plate

This selective agar is used at Udder Health Systems Laboratory for selection of all Staphylococci from bulk tank milk samples.

Modified Edwards Agar Plate

This is a special isolation agar used at Udder Health Systems Laboratory for selection of Streptococcus agalactiae from individual cow and bulk tank milk samples.

MP2 Agar

This is a selective isolation agar used at Udder Health Systems Laboratory for cultivating gram positive mastitis organisms from individual cow milk samples.

Mycoplasma Agar

This is the standard isolation agar used at Udder Health Systems Laboratory for cultivating Mycoplasma organisms from individual cow and bulk tank milk samples.

Prototheca Agar

This specialty selective agar is used for the cultivation of Prototheca sp. from individual cow milk, bulk tank milk or environmental samples.

TNT Agar

This biplate is used at Udder Health Systems Laboratory for selection and identification of both gram-positive and gram-negative organisms.

You may also download our full Culture Media Agars Brochure: Download (pdf)

Pricing Information

Agar Prices

Agar Quantity 1-99 100-399 400+
Camp $1.45/plate $1.20/plate $1.00/plate
Edwards $1.45/plate $1.20/plate $1.00/plate
Inulin $1.45/plate $1.20/plate $1.00/plate
MacConkey $1.45/plate $1.20/plate $1.00/plate
MP2 $1.45/plate $1.20/plate $1.00/plate
Salt $1.45/plate $1.20/plate $1.00/plate
Cow Blood $1.45/plate $1.20/plate $1.00/plate
Prototheca $2.45/plate $2.25/plate $2.00/plate
Mycoplasma $2.45/plate $2.25/plate $2.00/plate
TNT $2.45/plate $2.25/plate $2.00/plate
*The above agars may be combined in any quantity to achieve the price breaks.
 

Testing Supplies

Type Tube Bottle
Bovine Plasma
(Coagulase Test)
$1.00/Tube
(1mL)
100 mL: $40.00
200 mL: $60.00
500 mL: $120.00
Mycoplasma Broth
$1.00/Tube
(3mL)
500 mL: $60.00
 

Quality Control Organisms

Udder Health Systems is offering these organisms for Quality Control. Organisms are shipped out on agar plates Next Day Fed Ex.
Acholeplasma laidlawii $25.00 + Shipping
Trueperella pyogenes $25.00 + Shipping
Bacillus spp $25.00 + Shipping
E. coli $25.00 + Shipping
Klebsiella pneumoniae $25.00 + Shipping
Mycoplasma alkalescens $25.00 + Shipping
Mycoplasma bovis $25.00 + Shipping
Prototheca zopfii $25.00 + Shipping
Pseudomonas aeruginosa
$25.00 + Shipping
Pseudomonas spp $25.00 + Shipping
Staph. aureus $25.00 + Shipping
Staph. spp $25.00 + Shipping
Strep. agalactiae $25.00 + Shipping
Strep. dysgalactiae $25.00 + Shipping
Strep. uberis $25.00 + Shipping
Organisms are shipped out on agar plates via FedEx Next Day Air.
 

Shipping Information

We offer Shipping through the following Carriers:
Carrier Handling Options
FedEX Ground
2nd Day Air
Next Day Air
Saturday Delivery by Air
UPS Ground
2nd Day Air
Next Day Air
Saturday Delivery by Air
 
 
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