This is a selective isolation agar used at Udder Health Systems Laboratory for cultivating gram positive mastitis organisms from individual cow milk samples.
This cherry red, agar is prepared from a modified Columbia agar base with the addition of 5% washed bovine erythrocytes and full strength esculin. This selective agar has been formulated to enhance the growth of gram-positive staphylococci, streptococci and enterococci bacteria while inhibiting the growth of all gram- negative Enterobacteriaceae and Pseudomonas organisms. The addition of esculin enhances the capability of differentiating Streptococci. Certain Streptococci will hydrolyze esculin, causing a mild background blackening for E-strep and a mild greening or no background coloration for suspect Streptococcus agalactiae and Streptococcus dysgalactiae organisms. All suspect Streptococcus agalactiae organisms should be moved to our special Camp media for confirmation.
This is an indicator agar used for differentiating Streptococcus uberis from other E-streps in isolates from individual cow samples. The agar is also used as a selective agar in bedding samples for the isolation of Streptococcus uberis.