This is a specialized indicator agar used at Udder Health Systems Laboratory for confirmation of Streptococcus agalactiae suspects.
This cherry red, translucent agar is prepared from a blood agar base with the addition of 5% washed bovine erythrocytes and full strength esculin. This blood agar has been formulated to enhance the presentation of the Beta hemolysin zone of Staphylococcus aureus. The addition of esculin helps to distinguish Camp positive Streptococccus uberis from Streptococcus agalactiae. Certain Streptococci will hydrolyze esculin, causing a background blackening for E-strep and a mild greening or no background coloration for Streptococcus agalactiae and Streptococcus dysgalactiae organisms. Streptococcus agalactiae growth within the Beta hemolysin zone will exhibit the characteristic wedge shaped complete hemolysis of the CAMP reaction. All Camp positive, esculin negative organisms are recorded as Streptococcus agalactiae.
This is an indicator agar used for differentiating Streptococcus uberis from other E-streps in isolates from individual cow samples. The agar is also used as a selective agar in bedding samples for the isolation of Streptococcus uberis.