This is the standard isolation agar used at Udder Health Systems Laboratory for cultivating a wide range of mastitis organisms from individual cow and bulk tank milk samples.
This cherry red, translucent agar is prepared from a blood agar base with the addition of 5% washed bovine erythrocytes and ½ strength esculin. This blood agar has been formulated to enhance the detection of Staphylococcus aureus Alpha and Beta hemolysin when present. The addition of esculin enhances the capability of differentiating Streptococci. Certain Streptococci will hydrolyze esculin, causing a mild background blackening for E-strep and a mild greening or no background coloration for suspect Streptococcus agalactiae and Streptococcus dysgalactiae organisms. All suspect Streptococcus agalactiae organisms would be moved to our special Camp media. UHS blood agar will support good growth of a wide variety of fastidious microorganisms. When reading this agar; anticipate esculin hydrolysis on some coliforms as well.
This is an indicator agar used for differentiating Streptococcus uberis from other E-streps in isolates from individual cow samples. The agar is also used as a selective agar in bedding samples for the isolation of Streptococcus uberis.