Mycoplasma PCR Confirmation and Speciation

Why Species Matter

Udder Health Systems has maintained a long term commitment to providing state of the art dairy diagnostic products and services. As part of this commitment, we remain convinced that there are times in our industry when we can provide significant additional value to our laboratory customers, if more detailed species differentiation can be provided. Udder Health Systems believes that this is the case for Mycoplasma testing on cow milk.

Since the 1970’s the dairy industry has recognized that Mycoplasma mastitis presents a significant threat to dairy management with its alarming clinical mastitis rates, as well as expensive and cumbersome disease control requirements. Added to this threat was the annoying inevitability, that the industry standard culture detection method is slow (7-10 days incubation) and can produce “False Positives” in the form of the contaminant organism Acholeplasma. The Udder Health Systems Molecular Diagnostics Services now resolves these concerns, with a full spectrum of rapid PCR based Mycoplasma detection, confirmation and species identification methods.

This service is available to veterinarians, dairy consultants, or dairymen who wish to submit milk samples to UHS to look for Mycoplasma. It also available to other diagnostic laboratories who wish to use this as an out-lab service where “positive” culture plates or milk samples may be submitted for rapid testing. When submitting these samples, please select from our submission form, one of the following Test Codes:

128A: Mycoplasma Confirmation.
This test service is our standard mycoplasma agar culture confirmation service, to distinguish true positive Mycoplasma spp. from the “false positive” Acholeplasma spp. This test service replaces the old school “Digitonin Assay”. The 128A test has rapidly become a favorite with many of our long time customers. Gone are the days of phoning out a culture “positive” cow, and then having to wait another week to see if the test result confirms as a “True Positive” with the Digtionin method. Most of our customers now request automatic 128A confirmation on all myco agar growth so that we can finally eliminate the “false positive” reporting. If these plates are in the laboratory in the morning the confirmed result is available the same day.

128M7: Mycoplasma Speciation.
In addition to differentiating Mycoplasma spp. from Acholeplasma spp., this test will also identify if the isolate is one of the six most common mastitis causing species: M. bovis, M. bovigenitalium, M. californicum, M. canadense, M. arginini, M. alkalescens. These results are also available the same day, if the culture positive plate is in the laboratory in the morning.

125BP: Mycoplasma Broth PCR.
This service allows for the rapid detection of Mycoplasma in Bulk Tank or String samples. The PCR test gives a “same day” confirmed result from milk with no false positive Acholeplasmas, but also includes the slower and very sensitive conventional enriched agar culture. This 125BP test has proven very popular for fast detection of high count positives in “pooled” hospital group samples and in-line string samples where one would like to quickly follow up with individual cow testing if a group sample is PCR Myco positive. This rapid targeting system is used as part of a strategy to find “hot” cows quickly and dramatically lower the cost of Myco flare ups by getting high shedding cows out of the dairy significantly sooner.

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Meridian Laboratory
PCR and MALDI Services

Udder Health Systems P.C.
4455 S. Meridian Road
Meridian, ID 83642
P: 208-922-9505
F: 208-343-2046
Hours: 9:00am - 5:00pm MST (Mountain)

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